A new cell line established from calf kidney.

A new cell line “CK cell line” capable of continuous propagation was established from a calf kidney tissue. The bovine adenovirus type 3 could propagate well in this cell line. ∗PMID: 4280232 [PubMed indexed for MEDLINE] Copyright c ©OKAYAMA UNIVERSITY MEDICAL SCHOOL Acta Med. Okayama 28, 213-217 (1974) A NEW CELL LINE ESTABLISHED FROM CALF KIDNEY Makoto MOTO! Department of Pathology, Okayama University Medical School, Okayama, Japan (Director: Prof. K. Ogawa) Ginnohyoe SUHARA The Center for Adult Diseases, Kurashiki, Japan Received for publication, March 19, 1973 Abstract: A new cell line "CK cell line" capable of continuous propagation was established from a calf kidney tissue. The bovine adenovirus type 3 could propagate well in this cell line. A new cell line "CK cell line" capable of continuous propagation was established from a calf kidney tissue. The bovine adenovirus type 3 could propagate well in this cell line. Bovine adenovirus type 3 can induce undifferentiated tumors in adult hamsters as well as in newborn hamsters(l, 2). Since the tumor has characteristic features in tumor morphology and growth pattern, bovine adenovirus type 3-tumorigenesis needs to be studied in greater details. Cultured cell line, which is susceptible to this virus and is available at any time, is required for these studies. The authors have succeeded in establishing a calf kidney cell line for such a purpose. The present paper deals with the history of the cell line and some of its properties. MATERIALS AND METHODS Medium: Nutrient medium used for the primary and serial cultures was Eagle's minimum essential medium (MEM) supplemented with 20 per cent fetal bovine serum. Preparation of Primary Tissue Culture: A calf kidney was swabbed with 70 per cent alcohol and decapsulated. The kidney tissue was minced with scissors and washed twice with phosphate buffered saline (PBS). About 1 gm of minced tissue was suspended in about 100 ml of the solution containing an equal volume of O. 25 per cent trypsin and 0.5 per cent pancreatin, and stirred slowly at 4~C overnight for digestion. On the following day, cells were collected by centrifugation at 1,000 rpm for 5min, suspended in the growth medium, divided into bottles, stoppered and cultured at 37°C. Subculture: Bottles with vigorously growing cells were selected and used for further passages. Usually, three bottles were used for tissue cultures; two for a source of cell supply in continuous cultures and one kept in reserve. Nutient fluid was discarded, and cultured cells were quickly rinsed with PBS and treated with 0.1 per cent trypsin. Cells were collected by centrifugation